With frac30 fraction collector, automatically collect your pure protein in volumes ranging from 0. Protein purification laboratory of molecular biology. Aktafplc is a fully automated liquid chromatography system designed for. Akta purifier 10 fplc ge, amersham used, refurbished, service, repair.
Aktapurifiertm core systems fig 1 are versatile, modular. The ge amersham pharmacia akta pilot is a dedicated automated liquid chromatography lc system designed to carry out development, scaleup, and production processes. This manual describes the operation of the aktafplc system. Ge atka purifier 10 fplc system still in its originally factory seal and available at the lab world group.
The akta purifier 100 upc is a more economical choice than the akta purifier 100 ideally suited for applications that require 10 mil of flow rate per minute fast and easy start to purification work preprogramme. Information about these options can be found in optional configurations user manual which describes the extended functions of ae. Wash tubing with 20ml of proteins buffer at flow rate. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. I cleaned my column with naoh, water and then i equibrilated it with buffer.
This set up creates a lower flow rate but at a higher pressure required. It also features a tunable detector, allowing for selection of a wide spectral range. Akta pure 25 equipped with column selection valve v9c. Aktapurifier ge healthcare life sciences pdf catalogs. I was working with ge superdex 200 column on akta prime plus system. Suitable samples should be clarified prior to use with the system. A fact sheet has been prepared by the department which lists the components included with this system. The transferring department indicates that this unit is in working order. This biocompatible, hygienic and sterilizable system can purify products in milligrams of up to tens of grams. Back pressure increase may indicate treatment is overdue. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse.
Ge akta pilot liquid chromatography system gmi cerfied. Akta laboratoryscale chromatography systems sevier lab. Bioscisolutions repairs your akta, biacore, and agilent. The akta purifier 10 upc is the most economical choice of the 4 units.
This line of liquid chromatography systems is designed for fast and relia. Shutdown procedure for the akta purifier if you are not planning to continue your experiments the following day, proceed as follows. Akta laboratoryscale chromatography systems handbook. July 4, different optional configurations might be akta purifier 10 manual required, this is a modular fast performance liquid chromatography system used primarily for. Ge akta explorer certified fplc system gmi trusted lab. Appendix a akta purifier customized system scheme 1 iv908 buffer valve a 2 inv908 injection valve 3 pv908 precolumn 4 pv908 postcolumn 5 iv908 a18 tubingfalcon 6 pumpinjection valve waste bottle 7 20% etoh via b pump 8. Maintenance manually priming buffer inlet lines open draw air. The frac30 fraction collector supports four different tube sizes from 1. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. This automatic configuration protects the system from damaging events including over pressure. The high precision online monitor includes the possibility to. Using aktadesign automation kits to expand aktapurifier core systems also. Centrifuge or filter the samples prior sample loading, unless columns that.
When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template. This is a modular fast performance liquid chromatography system used primarily for fast separations and purifications of proteins, peptides and nucleic acids. Tuesday, august refurbished used lab, analytical, and biotech equipment. The ge akta purifier 100 is a fast protein liquid chromatograph or fplc, which is capable of handling a wide range of wavelengths. This section provides a short description of chapters 3 to 10. Cytiva formerly ge healthcare life sciences 2,012 views 2. Ge akta purifier 100 fplc comes preprogrammed with application protocols and method templates, making it a fast and easy start to purification work. Bufferprep kit eliminates the timeconsuming manual. Box monitor conductivity ph uv detector pumps injector buffers pump a buffer switching pump b valve online filter outlet valve mixer purifier. Aktapurifier upc 10 and 100 core systems include monitor upc900, which combines monitoring of uv absorption, ph, and conductivity. Experience improved versatility and tailormade solutions for specific application workflows by performing purifications at the microgram, milligram, or gram scale. The proven design has been verified during development and can be userconfigured to meet specific process demands.
Purification and online detection of cy5tm labeled cydyetm 20mer oligonucleotide. The akta purifier 10 can be simplified to be used as a nononsense workhorse or can be setup to navigate complex purification tasks. Akta chromatography systems have been a staple in protein purification for the past two decades and used throughout the globe. Ge healthcare akta fplc frac950 fraction collector frac950 10593 duration. Akta purifier 10 up to 10 mlmin flow rate at pressures of 0 to 25 mpa. Discussions about hplc, ce, tlc, sfc, and other liquid phase separation techniques. When using hazardous chemicals, all suitable protective measures, such as protective glasses, must be taken. For further details see akta avant user manual and akta pure user manual. Automatic self diagnostic tests check performance at every system start full biocompatibility ensures high recovery of biological activity an optimized flow path minimizes bandbroadening effects. Features include an automatic online buffer preparation from stock solutions, fixed volume loops up to 5 ml, super loops for 10 ml to 150 ml and quick solvent exchange without disrupting the. In the unicorn software, prompt the system control window.
Amersham akta purifier 10 w frac 950 recently sold the akta purifier is a high end liquid chromatography system with a wide range of preprogrammed application protocols and method templates used in academic and industrial research. Always keep the he inlet above the solution or close the valve. Ge akta purifier 10 with frac 920 recently sold the akta purifier is a high end liquid chromatography system with a wide range of preprogrammed application protocols and method templates used in academic and industrial research. Ge akta purifier 10 fplc system is verified by our industry trained service. It is ideally suited for applications which require up to 10 ml of flow rate per minute and where a filter based wavelength detector is adequate.
Before connecting valve inv907 ensure the power is switched off at the system pump or the complete system. This fplc has a capacity of up to 100 ml per minute. Ge akta purifier 10 fplc system marshall scientific. The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. A cooling function protects the fractions from heat degradation. Aktapurifier 10 and upc 10 configurations are designed for purification of. The akta purifier 100 comes preprogrammed with application protocols and method templates, making it a fast and easy start to.